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Description
Mouse TNF-α Culture Media& Cell culture extracts OneStep ELISA KitProduct Specification Antigen TNF Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Ms Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 2. 9% Inter assay: 6. 8% Sample type Cell culture supernatantCell culture extracts Assay type Sandwich (quantitative) Sensitivity 5. 055 pg mL Range 9. 38 pg mL 600 pg mL Recovery Cell culture supernatant:
Product Specification
| Antigen | TNF-α |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 2.9%； Inter-assay:6.8% |
| Sample type | Cell culture supernatant；Cell culture extracts |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 5.055 pg/mL |
| Range | 9.38 pg/mL – 600 pg/mL |
| Recovery | Cell culture supernatant: 84% Cell culture extracts: 83% |
| Assay time | 60 minutes |
| Species reactivity | Ms |
Background
Tumor necrosis factor α (TNF alpha) is an effective pro-inflammatory cytokine, which can kill and inhibit tumor cells. It is mainly produced by activated macrophages and can also be secreted by other types of cells, such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils and neuronal tissues. The members of TNF alpha family exert their cellular effect through two distinct surface receptors of the TNF receptor family, TNFR1and TNFR2. The pleiotropic biological effects of TNF can be attributed to its ability to simultaneously activate multiple signaling pathways in cells. TNF-induced NF-κB activation promotes the growth, invasion and metastasis of cancer cells.
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